Antibody reactivities ofMycobacterium paratuberculosisinfected sheep as analyzed by enzyme-linked immunosorbent assay and Western blotting
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چکیده
منابع مشابه
ENZYME-LINKED IMMUNOSORBENT ASSAY OF NEOPTERIN USING PENICILLINASE AS LABEL
An enzyme-linked immunosorbent assay for neopterin using penicillinase as marker enzyme is reported here by polyclonal antibodies against neopterin conjugated to bovine serum albumin which were raised in rabbits. Immunoglobulin fractions were purified and coated on wells of microtiter plates. A chain heterology was introduced in neopterin derivative and conjugated to penicillinase. The assa...
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Western blotting (WB) and enzyme-linked immunosorbent assay (ELISA) are the two most useful and sensitive methods to measure the ng/ml to pg/ml ordered materials in the solution, such as in tissue, serum, urine and cultured cells, etc, and they are especially widely used in protein detection. This article describes the principle theories and techniques for WB and ELISA procedure. [Ma H, Yang Y....
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There are increasing evidences that show many xenobiotic chemicals (called as endocrine disruptorchemicals EDCs) through interfering with endocrine system, have the capability to induce developmentaland reproductive abnormalities in humans and animals. The yolk protein precursor vitellogenin (VTG) hasproved to be a simple and sensitive biomarker for assessing exposure of fish to EDCs especially...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1991
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1991.tb04433.x